The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that can occur when performing conventional PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, realtime and quantitative PCR that are SYBR Green and probe based. The ZymoTaq™ PreMix yields specific amplicon formation with little or no byproducts. Simple and easy to use, just add water, primers, and template DNA to the ZymoTaq™ PreMix and then heat at 95oC for 10 minutes to initiate polymerization.ZymoTaq™ DNA polymerase is a heat-activated, hot start polymerase that has 3-terminal transferase activity. The addition of A overhangs to amplified DNA makes it ideal for use in TA-cloning.